Composite

Part:BBa_K3002229:Design

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 hygromycin resistance + cCA_WT-PETase_SP20His


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2868
    Illegal PstI site found at 3841
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2048
    Illegal PstI site found at 2868
    Illegal PstI site found at 3841
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1408
    Illegal XhoI site found at 250
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2868
    Illegal PstI site found at 3841
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2868
    Illegal PstI site found at 3841
    Illegal NgoMIV site found at 773
    Illegal NgoMIV site found at 955
    Illegal NgoMIV site found at 1326
    Illegal NgoMIV site found at 2603
    Illegal NgoMIV site found at 2630
    Illegal NgoMIV site found at 4332
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002119 (hygromycin resistance) and BBa_K3002124 (cCA_wildtype PETase_SP20His).

References