Composite
Part:BBa_K3002229:Design
Designed by: Marlene Schlosser Group: iGEM19_TU_Kaiserslautern (2019-10-21)
L2 hygromycin resistance + cCA_WT-PETase_SP20His
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2868
Illegal PstI site found at 3841 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2048
Illegal PstI site found at 2868
Illegal PstI site found at 3841 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1408
Illegal XhoI site found at 250 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2868
Illegal PstI site found at 3841 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2868
Illegal PstI site found at 3841
Illegal NgoMIV site found at 773
Illegal NgoMIV site found at 955
Illegal NgoMIV site found at 1326
Illegal NgoMIV site found at 2603
Illegal NgoMIV site found at 2630
Illegal NgoMIV site found at 4332 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
This part is a combination of the parts BBa_K3002119 (hygromycin resistance) and BBa_K3002124 (cCA_wildtype PETase_SP20His).